F4 FundFund
TeamPortfolioRaiseChampionsEssays
Sign in
Investment ThesisInvestment TeamF4 Research BriefSelected PortfolioRecent UpdatesFrequent co-investorsSimilar Firms
Hot Fund

Azolla Ventures

azollaventures.com · Cambridge, MA

“Impact-first investing in climate breakthroughs with the potential to reduce or remove at least 0.5 gigatons of CO2-equivalent emissions by 2050, targeting additional, overlooked ventures with a pathway to billion-dollar commercial success.”

Leads & Follows
Lead Tendency
$289M
AUM
01Investment Thesis

Impact-first investing in climate breakthroughs with the potential to reduce or remove at least 0.5 gigatons of CO2-equivalent emissions by 2050, targeting additional, overlooked ventures with a pathway to billion-dollar commercial success.

Exclusion Criteria

Ventures that could readily secure conventional venture funding (fail the additionality test), or that lack a credible path to gigaton-scale climate impact.

02Investment Team
AD
Amy Duffuor
Co-founder & General Partner
MN
Matthew Nordan
Co-founder & General Partner
JW
Johanna Wolfson
Co-founder & General Partner
AH
Ally Harada
Partner & Chief Operating Officer
AH
Af Hernandez
Principal
JM
John Mulliken
Operating Partner
EK
Erik Knall
Investment Associate
JC
Jillian Chase
Principal
EY
Eric Yao
Investment Analyst
NE
Nolan English
Head of Platform
CW
Carrie Winters
Executive Assistant
MS
Mariama Seydi
Climate Justice Advisor
03F4 Research Brief

Azolla Ventures Research

Investment Thesis

Azolla Ventures is an impact-first climate venture firm launched in 2021 by Prime Coalition, a nonprofit dedicated to steering capital toward scalable climate solutions. The firm is named for the "Azolla Event" - a geological period roughly 55 million years ago when the aquatic fern Azolla drew down atmospheric CO2 and reversed a warming climate - and frames its mission as enabling a planetary shift of similar magnitude by backing extraordinary teams commercializing climate breakthroughs. Every investment must clear three bars: impact (potential to reduce or remove at least 0.5 gigatons of CO2-equivalent emissions by 2050), additionality (unlikely to secure conventional venture funding at that stage), and commercial potential (a credible pathway to breakaway commercial success in a billion-dollar market). The firm has recently signaled a widening of its strategy to also address climate adaptation and resiliency, not just mitigation.

Stage Focus

Azolla invests at the earliest stages - pre-seed, seed, and seed-plus - where it argues risk and reward are highest and where catalytic capital can unlock deals conventional VCs pass on. It positions itself as a first institutional check for technically ambitious, capital-intensive climate ventures.

Check Size

Exact check size ranges are not disclosed publicly. Observed rounds range from ~$4M-$6M pre-seed/seed checks (e.g., Mafix's $5.4M pre-seed, FAST Metals' $4.3M raise) up to participation in much larger growth rounds for existing portfolio companies (e.g., Zanskar's $115M Series C).

Lead Tendency

Mixed. Azolla led Mafix's $5.4M pre-seed round in August 2026, indicating it will lead at the earliest stages, but it also participates alongside other climate-focused funds (Climate Capital, Skyriver Ventures, Silicon Badia, Spring Lane Capital, Obvious Ventures, Union Square Ventures, Lowercarbon Capital) in later, larger rounds for existing portfolio companies.

Recent Activity

Azolla has been actively deploying through mid-2026 across both of its vehicles - the $239M Azolla Fund I (closed May 2023) and the $50M Prime Impact Fund - for combined AUM of roughly $289M and 37 total investments to date. Recent activity includes: Mafix's $5.4M pre-seed (Aug 2026, led by Azolla), FAST Metals' $4.3M raise (Jul 2026), Eqon's $6M raise for smart heating-cable power management (Jun 2026), a JPMorgan carbon offtake and $20M debt financing deal for portfolio company Charm Industrial (Jun 2026), Meta's 100 GWh reservation with portfolio company Noon Energy (Apr 2026), Scalvy's $13.9M Series A for AI-data-center power delivery (Mar 2026), and Zanskar's $115M Series C for AI-powered geothermal exploration (Jan 2026).

Portfolio Highlights

Azolla's portfolio spans roughly 31 active companies across two funds. Fund I holdings include Heaten (industrial heat pumps), SiTration (battery recycling membranes), Funga (fungal microbiome carbon sequestration), Calcarea (shipping decarbonization), Scalvy (modular EV/grid power delivery), VELOZBIO (molecular-farmed alternative proteins), Muon Vision (critical metals), Aikido Technologies (floating offshore wind), Oxylus Energy (CO2-to-methanol conversion), Biolumic (light-based crop genetics), Still Bright (closed-loop copper extraction), Rock Zero (sustainable mining), Rain (autonomous wildfire-response aircraft), NetZeroNitrogen (biological nitrogen fixation), Erg Bio (sustainable aviation fuel), Dig Energy (low-cost geothermal heat pumps), EQON (efficient electrical industrial heating), FAST Metals (critical mineral recovery), and Mafix (CO2-removing silicon fertilizer). Prime Impact Fund holdings include Lilac Solutions (lithium extraction), Via Separations (membrane-based industrial separations), C-Motive Technologies (high-torque electric motors), Verdox (electroswing carbon capture), Gradient (efficient HVAC), Sublime Systems (electrochemical cement), Clean Crop Technologies (cold-plasma food-waste reduction), Charm Industrial (biomass-based carbon removal), Noon Energy (long-duration energy storage), Avalanche Energy (desktop-scale fusion), Zanskar (AI-driven geothermal exploration), Tynt (dynamic smart windows), and Vesta (coastal carbon capture via olivine weathering).

Team

  • Amy Duffuor - Co-founder & General Partner
  • Matthew Nordan - Co-founder & General Partner
  • Johanna Wolfson - Co-founder & General Partner
  • Ally Harada - Partner & Chief Operating Officer
  • Af Hernandez - Principal
  • John Mulliken - Operating Partner
  • Erik Knall - Investment Associate
  • Jillian Chase - Principal
  • Eric Yao - Investment Analyst
  • Nolan English - Head of Platform
  • Carrie Winters - Executive Assistant
  • Mariama Seydi - Climate Justice Advisor

The firm operates as a program of Prime Coalition, whose founder Sarah Kearney helped launch Azolla as Prime's dedicated early-stage investment vehicle.

Decision Process

Azolla combines a traditional partnership investment process (three co-founding GPs plus principals and an operating partner) with an independent impact-and-additionality assessment run through Prime Coalition, which gates both the initial investment and continued alignment on follow-on funding.

Founder Preferences

Azolla backs technically deep, often first-time or scientist-founder teams tackling capital-intensive, hardware- and process-heavy climate problems (industrial heat, mineral processing, carbon capture, geothermal, alternative proteins) that traditional software-oriented VCs typically avoid because of long R&D timelines and high capital intensity.

Geographic Focus

Primarily US-based (HQ in Cambridge, MA), but not strictly domestic - portfolio includes companies such as Norway's Eqon, indicating a willingness to back climate breakthroughs internationally when the impact and additionality case is strong.

04Selected Portfolio
AgTech & Agriculture
Biolumic·NetZeroNitrogen·Clean Crop Tech
Climate & Sustainability
Heaten·Funga·Scalvy·Oxylus Energy·Still Bright·Erg Bio·FAST Metals·Mafix·C-Motive Technologies·Verdox·Noon Energy·Avalanche Energy·Vesta
Consumer Apps & Social
Charm Industrial
Energy & Power
Rock Zero·Dig Energy·Lilac Solutions·Gradient Comfort·Zanskar Geothermal·EQON
Enterprise Software
Muon Vision·Sublime Systems
Fintech & Payments
Rain
Food & Beverage
VELOZBIO
Manufacturing & Industrial
SiTration·Via Separations·Tynt Technologies
Media & Entertainment
Aikido Technologies
Other
Calcarea
Open the full portfolio→
05Recent Updates

Fresh signals from public announcements, essays, and portfolio activity.

See full activity (7) →
Led Mafix's $5.4M pre-seed round to commercialize CO2-removing silicon fertilizers.
INVESTMENT·globenewswire.com
AUG 20, 2026
Backed Norwegian startup Eqon's $6M raise to reduce energy waste from electric heating cables.
INVESTMENT·azollaventures.com
JUN 24, 2026
Published a thesis essay on AI's overlooked water footprint alongside its energy demands.
ESSAY·azollaventures.com
JUN 6, 2026
Portfolio company Charm Industrial signed a carbon offtake deal and $20M debt financing with JPMorgan to expand carbon removal capacity.
MILESTONE·azollaventures.com
JUN 5, 2026
Participated in Scalvy's oversubscribed $13.9M Series A for AI data center, grid, and mobility power delivery.
INVESTMENT·prnewswire.com
MAR 26, 2026
06Frequent co-investors
PI
Prime Impact Fund
Both backed 12 of the same companies
Clean Crop Tech, Noon Energy, Zanskar Geothermal, Verdox,
+8 moreAvalanche Energy, C-Motive Technologies, Via Separations, Tynt Technologies, Vesta, Sublime Systems, Charm Industrial, Lilac Solutions
LC
Lowercarbon Capital
Both backed 4 of the same companies
Verdox, Sublime Systems, Charm Industrial, Lilac Solutions
EV
Engine Ventures
Both backed 3 of the same companies
Via Separations, Sublime Systems, Lilac Solutions
I
Incite
Both backed 3 of the same companies
Dig Energy, Gradient Comfort, Charm Industrial
AV
Avila VC
Both backed 2 of the same companies
Dig Energy, Tynt Technologies
Find the investors for your round→
07Similar Firms
BE
Breakthrough Energy Ventures
$500K – $50M · Seed · Series A
→
CE
Clean Energy Ventures
$500K – $15M · Pre-Seed · Seed
→
PI
Prime Impact Fund
$1M – $4M · Seed · Series A
→
BF
Baruch Future Ventures (BFV)
$250K – $10M · Pre-Seed · Seed
→
PV
Prelude Ventures
$100K – $5M · Pre-Seed · Seed
→
Quick Reference
Stages
Pre-Seed · Seed
AUM
$289M
Decision
Partnership
Location
Cambridge, MA
Sector Focus
Manufacturing & IndustrialClimate & SustainabilityFood & Beverage
Models
Climate TechDeep Tech
Technology
HardwareCarbon CaptureEnergy Storage
See all Manufacturing & Industrial investors →See all Climate & Sustainability investors →See all Food & Beverage investors →
Visit azollaventures.com ↗

See how Azolla Ventures matches your startup

Get a personalized match report in minutes.

Get Your Match Report
Focus Areas
Manufacturing & IndustrialClimate & SustainabilityFood & Beverage

See how Azolla Ventures matches your startup

Get a personalized match report in minutes.

Get Your Match Report
Is this your firm? Claim your profile
F4 Intelligence Report · Last Researched August 22, 2026F4 Research Engine