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Threshold Ventures

thresholdventures.com · Palo Alto, CA

Investment thesis

Back founder-led, category-defining companies at moments of market transformation, early and with active board partnership.

Leads
Lead Tendency
01Investment Thesis

Back founder-led, category-defining companies at moments of market transformation, early and with active board partnership.

02Investment Team
EM
Emily Melton
Managing Partner
JS
Josh Stein
Managing Partner
AM
Arpit Mittal
Partner
HR
Heidi Roizen
Partner
LX
Lisa Xu
Partner
AS
Andreas Stavropoulos
Partner
JK
Jen Kodner
Talent Partner
03F4 Research Brief

Threshold Ventures Research

Investment Thesis

Threshold is a small, founder-focused early-stage firm in Palo Alto. Its stated approach is to partner with founders building category-defining companies during moments of market transformation, and to back them before their breakthrough. The site says it invests in founders with deep expertise and unique insights, in markets undergoing transformation, and in enabling technology adoption. It describes itself as small by design, which it says lets it stay nimble, invest with conviction and act as an active board member rather than a passive check.

Origins and Fund History

The firm was founded by Emily Melton and Josh Stein, who have worked side by side for more than two decades. It began as the venture practice of DFJ Growth and was spun out as an independent firm, rebranded Threshold Ventures in 2019. Funds named in public sources include Threshold IV and Threshold Select, announced about four years ago, and Fund V, reported at $250 million closing in 2023. A Form D for Threshold Ventures V, L.P. is listed in a public startup funding database with a Palo Alto address.

Stage Focus

The about page says the firm primarily invests in Seed to Series B companies. Its recent investments run from seed AI labs to Series A and B rounds, and the long-tenured portfolio includes several companies that went public.

Check Size

Threshold does not publish check sizes or fund size on its site. No figure is given here; it would be inferred from round sizes only.

Lead Tendency

The firm leads rounds. It led Suger's $15M Series A in February 2025 and Brightline's Series A with Oak HC/FT, and Josh Stein led Box's Series A in 2006. It also takes board seats and describes itself as committed to active investing, helping founders through make-or-break decisions at every stage.

Recent Activity

  • Suger raised a $15M Series A in February 2025 led by Threshold, with Craft Ventures, Intel Capital and Y Combinator.
  • Sequen announced a $90M Series B in September 2026 led by Prysm Capital; Threshold is reported among the participants.
  • The portfolio page lists 2026 investments in Bluefish, Core Automation, Enclave, Interia and Autopoiesis, and 2025 investments in Adaption, Attuned Intelligence, Daero, Laborup, Moonlake and M7 Health.

Portfolio Highlights

Exits and public companies include Box (NYSE: BOX, invested 2006), Doximity (NYSE: DOCS), Livongo (Nasdaq: LVGO, acquired by Teladoc), Redfin, Remitly, Twilio, Epocrates, Rippling (private), and the acquisitions Nervana (Intel), OODA Health (Cedar), Chef (Progress Software), BentoBox (Fiserv), Chartbeat, CrowdAI (Saab), Divvy Homes (Maymont Homes), Loftium (Flyhomes) and Lumity (Alera Group). Active companies include BetterUp, LaunchDarkly, Front, CircleCI, Databook, CloudZero, Freeform, Mendaera, Imbue and Sequen. The portfolio is concentrated in enterprise software, developer tools, digital health and AI.

Team

  • Emily Melton, Managing Partner
  • Josh Stein, Managing Partner
  • Arpit Mittal, Partner
  • Heidi Roizen, Partner
  • Lisa Xu, Partner
  • Andreas Stavropoulos, Partner (board)
  • Jen Kodner, Talent Partner
  • Johnna Lesch, CFO

Decision Process

A public profile says Melton and Stein share decision-making authority with equal voting power on the investment committee, so the decision process is a partnership. Founders on the site describe fast, personal involvement from the managing partners.

Founder Preferences

Founder testimonials stress deep personal involvement, humility, and experience at scale. The firm favors founder-led companies with unique insight into a transforming market, often technical or domain experts, such as the physician-founder style of Doximity and the operator founders of Brightline and Databook.

Geographic Focus

The firm is based in Palo Alto, California. Portfolio companies are mostly in the United States, with some international companies such as Balena, ODAIA and Numa, so geography is not a stated constraint.

Gaps

AUM, check size and warm-intro requirements are not stated on the site. The portfolio page text was partly truncated in the read, so the later alphabet was filled from image captions. Domains are left null for all portfolio companies.

04Selected Portfolio
Biotech & Life Sciences
Atomwise·Moonlake·Probably Genetic
Developer Tools & Infrastructure
CircleCI·Nervana
E-commerce & Marketplaces
Bluefish
Enterprise Software
Adaption·AutoLeap·Autopoiesis·balena·Box·Chartbeat·Chef·CloudZero·Core Automation·CrowdAI·Daero·Databook·Diamanti·EdgeQ·Elementary·Enclave·Forward Networks·Front·HumanFirst·Imbue·Instil·LaunchDarkly·Odaia·OpsLevel·Rippling·Twilio·Sanity·Periscope Data·Sequen·SugarCRM·Talkdesk·Veritus·Suger
Fintech & Payments
LendKey·Remitly
Healthcare & Digital Health
Attuned Intelligence·Calibrate·Doximity·Elation Health·ePocrates·Imagen·Livongo·M7 Health·Mendaera·OODA Health·Transcarent·Ritten·Vital·Xealth
HR Tech & Future of Work
Benepass·BetterUp·ChangeEngine·Laborup·Lumity·Numa
Legal & Regtech
Spellbook
Logistics & Supply Chain
Brightline
Manufacturing & Industrial
Motion
Marketing & Adtech
Freshpaint
Media & Entertainment
BentoBox·Freeform
Real Estate & PropTech
Divvy Homes·Interia·Loftium·Redfin
Robotics & Automation
Mythic
Security & Cybersecurity
Inscribe
Other
AngelList·Mindshow·Parasail
Open the full portfolio→
05Recent Updates

Fresh signals from public announcements, essays, and portfolio activity.

Portfolio company Sequen announced a $90M Series B led by Prysm Capital.
MILESTONE·cooley.com
SEP 2026
Led Suger's $15M Series A for cloud marketplace operations.
INVESTMENT·finsmes.com+1
FEB 2025
06Frequent co-investors
SV
Sapphire Ventures
Both backed 4 of the same companies
Box, BetterUp, CircleCI, Livongo
7V
7wire Ventures
Both backed 3 of the same companies
Brightline, Transcarent, Livongo
CH
Corewell Health Ventures
Both backed 3 of the same companies
Brightline, Transcarent, Livongo
CC
Crosslink Capital
Both backed 3 of the same companies
Inscribe, Veritus, BetterUp
H
Heavybit
Both backed 3 of the same companies
Sanity, CircleCI, LaunchDarkly
Find the investors for your round→
Quick Reference
Stages
Seed · Series A · Series B
Decision
Partnership
Involvement
Board Seat
Location
Palo Alto, CA
Sector Focus
Fintech & PaymentsEnterprise SoftwareDeveloper Tools & InfrastructureHealthcare & Digital Health
Models
B2B SaaSDeveloper ToolsDigital Health
Technology
AI/ML
See all Fintech & Payments investors →See all Enterprise Software investors →See all Developer Tools & Infrastructure investors →
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Focus Areas
Fintech & PaymentsEnterprise SoftwareDeveloper Tools & InfrastructureHealthcare & Digital Health

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F4 Intelligence Report · Last Researched October 31, 2026F4 Research Engine